Care
of Carbon Fiber Rotor
Carbon fiber rotors
are extremely durable and designed to give many years of continuous
service in all types of laboratory. This article describes the
procedures that should be used to ensure the long life of your
rotor which has a Lifetime Warranty.
The most important
procedure for all rotors is to ensure that the rotor is cleaned
regularly after use. This is essential not only for safety
purposes but also to maximize the life of the rotor. Particular
care must be taken in cleaning and decontaminating rotors after
they have been used with hazardous materials such as radioisotopes
or infectious organisms and these aspects are dealt with in later
sections of this article. While carbon fiber rotors are not affected
by the corrosive action of salts, all rotors contain some metal
components and these can corrode depending on the exact alloy
present. Hence, all rotors should be cleaned on at least a daily
basis.
Routine washing of rotors
Unlike metal rotors,
carbon fiber rotors can be washed with strong laboratory detergent
solutions, even alkaline detergents. Rotors can be either washed
in detergent solutions or, if necessary, the rotor pockets can
be filled with detergent solution and left overnight to remove
any resistant deposits. We recommend the use of FIBERClene cleaning
agent since this has been specially formulated for cleaning carbon
fiber material and is very safe environmentally. It is important
to note that the metal parts of the rotor should not be exposed
to detergent solution for any prolonged length of time as otherwise
they may corrode. If deposits of the material have accumulated
in the rotor then it may be necessary to use a soft brush to dislodge
them from the surface. Scratches will affect the cosmetic appearance
of the rotor but, unlike metal rotors, scratches do not affect
the chemical resistance of the rotor material in any way.
Most radioisotopes
can be readily removed from FIBERLite CENTRIFUGE
rotors because they do not usually bind to the surfaces of composite
rotors in the same tenacious way as they do to metallic rotors.
Hence, carbon fiber rotors are much easier to decontaminate than
metallic rotors. There are a number of commercially available
detergents for removing radioisotopes from centrifuge rotors and
these can be used for decontaminating FIBERLite CENTRIFUGE
rotors. In addition, most general laboratory detergents can be
used to remove radioisotopes from rotors without harming the carbon
fiber material or the rotor finish. However, we would recommend
FIBERClene that is a cleaning agent specially formulated for FIBERLite
CENTRIFUGE rotors. In most cases, radioisotopic contamination
can be removed simply by wiping the affected part of the rotor
with a tissue soaked in the cleaning agent or detergent. In the
case of high levels of contamination, it may be necessary to soak
the rotor overnight to achieve complete removal of the radioisotope.
Disinfecting procedures for
removing infectious agents
Infectious agents
can be broadly classified into fungi, bacteria, viruses, and prions.
Centrifuge rotors may become contaminated with one or more of
these infectious agents depending on the application that the
rotor be being used for. It is essential that after any work with
samples that may be associated with infectious agents, the rotor
is immediately disinfected before further use. When the rotor
is regularly used with infectious agents, a clear schedule of
decontamination should be instituted and the rotor should be clearly
marked as being likely to be contaminated with biohazards material.
Disinfection of rotors can be carried out in a number of different
ways. However, some methods, for example UV light irradiation
are not very practical for centrifuge rotors. In other cases,
the chemical susceptibility of rotor material(s) restricts the
type of disinfection process that can be used. For example, sterilization
with ethylene oxide vapor or disinfection with 10% hydrogen peroxide
are not recommended for FIBERLite CENTRIFUGE carbon
fiber rotors. In contrast, the use of 0.1M sodium hydroxide, recommended
for inactivating prions, is compatible with carbon fiber rotors
but not with aluminum rotors. The final choice of disinfection
used for the rotor depends primarily on the nature of the infectious
agent thought to be contaminating the rotor. For example, in terms
of disinfection by heat treatment, relatively low temperatures
of about 60° C while bacterial spores are very heat resistant
and are only killed inactivate many viruses by autoclaving. In
fact, autoclaving is the preferred method for disinfecting rotors
because it does not involve any chemicals, some of which are harmful
and even suspected carcinogens. When using chemical disinfection
it is essential to ensure that all parts of the rotor are treated,
preferably for at least 30 minutes, although some infectious agents
may require treatment for up to 10-12 hours for complete sterilization.
The table below summarizes the disinfection properties of various
procedures.
| Sterilization
or disinfection agent |
Fungi |
Bacterial cells |
Bacterial spores |
Lipid viruses |
Non-lipid viruses |
Prions |
| Autoclaving (121°
C)1 |
++++ |
++++ |
++++ |
++++ |
++++ |
++++ |
| Formaldehyde (37%) |
++++ |
++++ |
++++ |
++++ |
++++ |
-- |
| Glutaraldehyde
(2%)2 |
++++ |
++++ |
++++ |
++++ |
++++ |
+++ |
| 70% ethanol |
+ |
++++ |
-- |
++++ |
+ |
-- |
| Phenolic solutions3 |
++++ |
++++ |
+ |
++++ |
++ |
-- |
| 1 15psi
(1 bar) for 15 minutes |
++++ Recommended
|
| 2 Commercially
available as Cidexâ |
+++ Probably effective
in most cases |
| 3 Commercially
available as Lysolâ |
++ Effective in
some cases |
| |
+ limited use |
The recommendations given here are only for guidance and therefore
before implementing any of these procedures the user should verify
that they are appropriate by consulting the appropriate reference
sources.
Inspection of
rotors
Your FIBERLite
CENTRIFUGE rotor has been designed to last a lifetime and
provided that the rotor has been used correctly it should not
require any maintenance other than routine cleaning. However,
if it is possible that the rotor has been overstressed by, for
example, centrifuging samples denser than 1.2 g/ml without derating
the maximum speed of the rotor or if the rotor has been dropped
onto a solid surface (e.g. concrete) from a height of 1.0m (3ft)
or more sufficient to mark the outside of the rotor then it should
be returned to the factory for specialist inspection.
|